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Molecular Evolutionary Genetics Analysis (MEGA) is computer software for conducting statistical analysis of molecular evolution and for constructing phylogenetic trees. It includes many sophisticated methods and tools for phylogenomics and phylomedicine. It is licensed as proprietaryfreeware. The project for developing this software was initiated by the leadership of Masatoshi Nei in his laboratory at the Pennsylvania State University in collaboration with his graduate student Sudhir Kumar and postdoctoral fellow Koichiro Tamura.^[2] Nei wrote a monograph (pp. 130) outlining the scope of the software and presenting new statistical methods that were included in MEGA. The entire set of computer programs was written by Kumar and Tamura. The personal computers then lacked the ability to send the monograph and software electronically, so they were delivered by postal mail. From the start, MEGA was intended to be easy-to-use and include solid statistical methods only.

MEGA version 2 (MEGA2), which was coauthored by an additional investigator Ingrid Jakobson, was released in 2001.^[3] All the computer programs and the readme files of this version could be sent electronically due to advances in computer technology. Around this time, the leadership of the MEGA project was taken over by Kumar (now at Temple University) and Tamura (now at Tokyo Metropolitan University). The monograph Molecular Evolutionary Genetics Analysis was often used as a textbook for new ways to study molecular evolution.

MEGA has been updated and expanded several times and currently all these versions are available from the MEGA website. The latest release, MEGA7, has been optimized for use on 64-bit computing systems. MEGA is in two version. A graphical user interface is available as a native Microsoft Windows program. A command line version, MEGA-Computing Core (MEGA-CC), is available for native cross-platform operation. The method is widely used and cited. With millions of downloads across the releases, MEGA is cited in more than 85,000 papers. The 5th version has been cited over 25,000 times in 4 years.^[4]

Release history[edit]

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• Media Creation Tool 21H1 (10.0.19041.572) on 32-bit and 64-bit PCs. This download is licensed as freeware for the Windows (32-bit and 64-bit) operating system on a laptop or desktop PC from disk management without restrictions. Media Creation Tool 21H1 (10.0.19041.572) is available to all software users as a free download for Windows.

Airwell Mega Tool Software; Manuale D`uso Mercedes Ml 320 Cdi; Digitech Rp500 Patch Library; Microsoft Toolkit 2.4.7 (Activation Office 2013 Windows 8).

Features[edit]

Sequence alignment construction[edit]

• Alignment editor
• Sequencer (trace) file editor-viewer
• Integrated web browser, sequence fetching

Data handling[edit]

• Handling ambiguous states: R, Y, T, etc.
• Extended MEGA format to save all data attributes
• Importing data from other formats: Clustal, Nexus, etc.
• Data explorers
• Visual specification of domains-groups

Genetic code table section[edit]

• Add-edit user defined tables
• Compute statistical attributes of a code table
• Include all known code tables

Real-time caption expert engine[edit]

• Generate captions for distance matrices, phylogenies, tests, alignments
• Copy captions to external programs

Integrated text file editor[edit]

• Columnar block selection-editing
• Line numbers
• Utilities to format sequences, reverse complement etc.

Sequence data viewer[edit]

• Data export
• Highlighting
• Statistical quantities estimation

MCL-based estimation of nucleotide substitution patterns[edit]

• 4x4 rate matrix
• Transition-transversion rate ratios: k1, k2
• Transition-transversion rate bias: R

Substitution pattern homogeneity test[edit]

• Composition distance
• Disparity index
• Monte-carlo test

Distance estimation methods[edit]

• Nucleotide-by-nucleotide
• Synonymous-nonsynonymous: codon-by-codon
• Protein distance
• Distance calculations
• Sequence diversity calculations
• Variance calculations

Tests of selection[edit]

• Large sample Z-test
• Fisher’s exact test
• Tajima’s neutrality test

Molecular clock test[edit]

• Tajima’s relative rate test

Tree-making methods[edit]

• Minimum evolution method
• UPGMA
• Bootstrap phylogeny test
• Confidence probability test
• Distance matrix viewer

Tree explorers[edit]

• Phylogeny display
• Divergence time estimation
• Tree editing
• Change tree size
• Multiple tree display

External links[edit]

References[edit]

1. ^ ^ab'Update History'. megasoftware.net. Retrieved 2021-04-19.
2. ^ ^abKumar, S., K. Tamura, and M. Nei (1993) MEGA: Molecular Evolutionary Genetics Analysis. Ver. 1.0, The Pennsylvania State University, University Park, PA.
3. ^ ^abcKumar, S., K. Tamura, I. B. Jakobsen, and M. Nei (2001) MEGA2: Molecular Evolutionary Genetics Analysis. Ver. 2.0, Bioinformatics 17:1244-1245.
4. ^ ^abTamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol. 30:2725-2729.
5. ^'MEGA Update History'. MEGA: Molecular Evolutionary Genetics Analysis. Retrieved 20 June 2013.
6. ^Kumar, S., K. Tamura, and M. Nei (1994) MEGA: Molecular Evolutionary Genetics Analysis software for microcomputers. CABIOS 10:189-191.
7. ^Kumar, S., Tamura, K., and Nei, M. (2004) MEGA3: Molecular Evolutionary Genetics Analysis. Brief. Bioinformatics 5:150-163.
8. ^ ^abTamura, K., Dudley, J., Nei, M., Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis. Mol. Biol. Evol. 24(8):1596-1599.
9. ^ ^abcTamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. 28:2731-2739.
10. ^Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33(7):1870–1874.
11. ^Kumar, S., Stecher, G., Li M., Knyaz C. & Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol. Biol. Evol. 35(6):1547-1549.

The newest ODIS software has been updated to V5.26 which works with VAS6154 and VAS5054A on Win 7 & Win 10 32 bit/64 bit.

What’s new in ODIS 5.26?

(1)ODIS Software Version: 5.26 for Full Brand till 2019.12 (also includes 5.16 for each brand till 2019.8)

(2)ETKA: 8.0 (2018.11)

(3)Elsawin: 6.0 (2016.1)

(4)ODIS Engineering: 11.0

(5)VCDS Software Version: 19.6.2

(6)OS System: Win7, Win10 32bit/ 64bit

The old software version all come with win7 system, most of customers know that the VAS5054A doesn’t support to use with win10 system, but with this 5.26 version is ok to use on Win10.

(7)Multi-Languages:

English/Japanese/Portuguese/Romanian/Korean/Dutch/Polish/Turkish/Spanish/French/Italian

Croatian/Danish/German/Swedish/Finnish/Slovenian/Czech/Russian/Greek/Chinese

(8)Support Vehicles: VAG Group includes Volkswagen (Shanghai VW, FAW-VW, VW Commercial Vehicles etc.), Audi, Lamborghini, Bentley, Skoda, Seat, Bugatti, MAN total 11 brands.

(9)Compatible Hardware: VAS5054A (Item No.SP33-B, SP33-BO), VAS6154A

Where to download ODIS 5.26 Postsetup 98.0.160?

The download links offered below are testing ok, no dead links.

Torrent 1

Size: 18KB
0.MS_Visual_C++2010 for W10
0.Prerequirements x64 W7
0.Prerequirements x86 W7
1.OffboardDiagSetup-Service_VWMCD_5_2_6-B52_6_0_2
2.ODIS-S 5.2.6 Patch+License-Full After Unrar this the password for the 7zip archive is as alwaysfantomel
3.PDU-API_fix_x86+x64 for W10
5.Bad VAS5054A Patch
5a.Softing_PDU-API_1.20.42 for clones
EN SFD-Manual for UMB.pdf
EN Windows 7 Announcement UMB.pdf
supportnews-en.pdf

Mega Tool Academy

Torrent 2
4.ODIS-Service_update_5_2_6-EU_20200317_98.0.140

Size: 44KB

Torrent 3
ODIS-Service_update_5_2_6-EU_20200330_98.0.160

Size: 39KB

Torrent 4
4a.ODIS-Service_update_5.1.6_v89.5.520 to 5.2.6-EU_20200303_96.0.10

Size: 16KB

Torrent 5
4b.ODIS-Service_update_5.1.6_v89.5.520 to 5.2.6-EU_20200317_98.0.140

Size: 15KB

Win10-VAS5054-ODIS-S-526:

How to install VAS 5054A, VAS 6154 ODIS 5.26?

Here is the installation guide on Win7 32 bit for reference.

The installation guide for VAS5054a and VAS-6154 are same. The difference is that it requires to install VAS driver for VAS6154.

VAS 5054A ODIS 5.26 install video

VAS 6154 ODIS 5.26 install video

Note: It will take a long time to copy the 5 software from DVD to the local disk of computer, please keep patient.

Main steps:

Step 1: Install OffboardDiagSetup-service_VWMCD_5_2

Step 2: Install Offboarddiaglauncher

Step 3: Run Offboard Diagnostic information system

Step 4: Install ODIS 5.26 patch

In detail…

Depends on your need to update windows system

Step 1: Install OffboardDiagSetup-service_VWMCD_5_2

Select a language

Choose target folder

Select diagnostic interface: VAS 5054 or VAS 6154

Click “Search” to select the related license

Install Offboard Diagnostic information system service success

Step 2: Install Offboarddiaglauncher

Copy the offboardDiagLauncher from local disk to program files (x86)

Step 3: Run Offboard Diagnostic information system

Right click “Offboard Diagnostic information system” to run as administrator

Open “keygen” option

Get the hardware ID and name from “Offboard Diagnostic information system

5.23 Registration Dialog” to the corresponding positions in the “Keys Generator”

Then obtain the registration key and paste to “Registration Dialog” to register

Input Postsetup configuration data

URL: Click “Select local directory” to choose

User name: abc

Kerword: abc

Select the corresponding language and download ODIS data

Restart computer

Step 4: Install ODIS 5.26 patch

Copy ODIS 5.26 patch to C disk

Mega Tool Drills

Install ODIS 5.26 software succeed

Then you can start to diagnose…

ODIS 5.2.6 Postsetup 98.0.210 tech support: www.cardiagtool.co.uk

Airwell Mega Tool Software Update